hela wt cells (ATCC)

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ATCC hela wt cells
Hela Wt Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 16375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hela+cells/HeLa/pm42321163-224-4-2
Average 99 stars, based on 16375 article reviews

hela wt cells - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: Single-cell RNA sequencing profiles drug activity within spatially engineered 3D cultures.
Article Snippet: .. HeLa cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco 10569044) supplemented with 10% v/v FBS at 37 °C and 5% CO2. .. HeLa cells were maintained at 37 °C in a humidified incubator with 5% CO2 and passaged at 70–80% confluency using 1× TrypLE Express (Gibco).

Article Title: Targeted RNA editing
Article Snippet: .. HeLa cells (ATCC, CCL-2) are cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum. ..

Article Title: Enhanced Efficacy of EGFR-Targeted ADCs via p38-Mediated Noncanonical Endocytosis in EGFR-Mutant Lung Cancer Cells.
Article Snippet: They were cultured in RPMI-1640 (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% fetal calf serum (FCS), 2 mM l-glutamine, 100 U/mL penicillin, and 100 U/mL streptomycin (Meiji Seika Pharma, Tokyo, Japan) at 37°C in 5% CO2. .. HeLa cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, U.S.A.) and cultured in Dulbecco’s modified Eagle’s medium (Nissui Pharmaceutical) supplemented with 10% FCS, 4 mM l-glutamine, 100 U/mL, and 100 U/mL streptomycin at 37°C in 5% CO2. .. Antibodies and Reagents Antibodies against EGFR (C74B9), phospho-EGFR (Tyr-1068), and phospho-p38 (Thr180/Tyr-182) were obtained from Cell Signaling Technology (Danvers, MA, U.S.A.).

Modification:

Article Title: TRK-T3 condensate organization drives growth signaling.
Article Snippet: .. HeLa cells (CCL2; ATCC) and HEK293T (3216; ATCC) cells were grown at 37°C at 5% CO2 in Dulbecco’s modified eagle medium (cat #10566-016; Gibco) supplemented with 10% FBS [A31604-01, A5669402; Gibco]. .. Cells were seeded on plain glass-bottom or collagen-coated glass-bottom Mattek dishes (P35GC-1.5-14-C, P35GCOL-1.5-14-C) for imaging.

Isolation:

Article Title: Anti-CCR8 antibodies
Article Snippet: Many mammalian cell lines suitable for expression of heterologous polypeptides are known in the art and can be obtained from a variety of vendors including, e.g., American Type Culture Collection (ATCC). .. Suitable mammalian host cell lines include, for example, the COS-7 line (ATCC CRL 1651) (Gluzman et al., (1981), SV40-transformed simian cells support the replication of early SV40 mutants, Cell 23 (1): 175-182), L cells, C127 cells, 3T3 cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, or their derivatives such as Veggie CHO and related cell lines, which grow in serum-free media (Rasmussen et al. (1998), Isolation, characterization and recombinant protein expression in Veggie-CHO: a serum-free CHO host cell line, Cytotechnology 28:31), CHO-K1 and CHO pro-3 cell lines and their derivatives such as the DUKX-X11 and DG44 cell lines, which are deficient in dihydrofolate reductase (DHFR) activity, Hela cells, baby hamster kidney (BHK) cells (e.g., ATCC CRL 10), the CVI/EBNA cell line derived from the African green monkey kidney cell line CVI (ATCC CCL 70) as described by McMahan et al. (1991), A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types, EMBO J. .. 10 (10): 2821-2832, human embryonic kidney (HEK) cells such as HEK 293, 293 EBNA or MSR 293, human epidermal A431 cells, human Colo205 cells, HL-60 cells, U937 cells, Hak cells, Jurkat cells, HepG2/3B cells, KB cells, NIH 3T3 cells, S49 cells, PER.C6 (Crucell), CAP and CAP-T cells (CEVEC), and mouse myeloma cells, including NSO and Sp2/0 cells.

Recombinant:

Expressing:

Article Title: Method of treating psoriatic arthritis patients with inadequate response to TNF therapy with anti-IL23 specific antibody
Article Snippet: .. A number of suitable host cell lines capable of expressing intact glycosylated proteins have been developed in the art, and include the COS-1 (e.g., ATCC CRL 1650), COS-7 (e.g., ATCC CRL-1651), HEK293, BHK21 (e.g., ATCC CRL-10), CHO (e.g., ATCC CRL 1610) and BSC-1 (e.g., ATCC CRL-26) cell lines, Cos-7 cells, CHO cells, hep G2 cells, P3X63Ag8.653, SP2/0-Ag14, 293 cells, HeLa cells and the like, which are readily available from, for example, American Type Culture Collection, Manassas, Va. (www.atcc.org). ..

Activity Assay:

Derivative Assay:

Clone Assay:

Retroviral:

Article Title: CTL peptide epitopes and antigen-specific T cells, methods for their discovery, and uses thereof
Article Snippet: .. Platinum-E (Plat-E) retroviral packaging cell line (referred to as HEK) (Cell Biolabs, San Diego, CA, USA) and HeLa cells were maintained in DMEM (Invitrogen); MSC, HaCaT and HCT-116 were grown in D-MEM/F12 (Sigma); Caco-2 and Hep G2 in EMEM (ATCC®), all with 10% FCS and penicillin/streptomycin (P/S). .. The other cell lines were cultured in RPMI 1640 (Gibco), with 10% FCS and P/S.

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